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KMID : 1025520000420060941
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Journal of Animal Science and Technology
2000 Volume.42 No. 6 p.941 ~ p.956
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Inactivation of the Whey Acidic Protein(WAP) Gene by Site - Specific Recombination in Mouse Embryonic Stem Cells
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Abstract
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Regulatory elements of the whey acidic protein (WAP) gene are widely used for the expression of transgenes into milk. Although high levels of transgene expression can be obtained using the WAP promoter, there can be problems in these transgenic animals such as abnormal development of the mammary gland. Towards the understanding of its function, the mouse WAP gene in embryonic stem (ES) cells was inactivated using a combination of homologous recombination and site-specific recombination. The endogenous locus was successfully targeted with a construct containing three loxP sites. After transient expression of Cre recombinase, two types of deletion events were seen in the targeted WAP locus, a complete deletion of the WAP gene and the neomycin-thymidine kinase (Neo-TK) selection cassette (type I deletion), and a deletion of the selection cassette only leaving the intact WAP gene flanked by two loxP sites (type II deletion). Both of these cell lines should facilitate the study of the physiological function of the WAP.
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KEYWORD
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